SuicidDentistV1, Main, Exploration, bibRecord, 000135

The effect of mesoporous bioglass on osteogenesis and adipogenesis of osteoporotic BMSCs.

Identifieur interne : 000135 ( Main/Exploration ); précédent : 000134; suivant : 000136

The effect of mesoporous bioglass on osteogenesis and adipogenesis of osteoporotic BMSCs.

Auteurs : Tao Wu [République populaire de Chine] ; Ning Cheng [République populaire de Chine, États-Unis] ; Chun Xu [Australie] ; Wei Sun [République populaire de Chine] ; Chengzhong Yu [Australie] ; Bin Shi [République populaire de Chine]

Source :

Journal of biomedical materials research. Part A [ 1552-4965 ] ; 2016.

RBID : pubmed:27449696

Descripteurs français

KwdFr :
- Adipogenèse (MeSH), Animaux (MeSH), Cellules cultivées (MeSH), Cellules souches mésenchymateuses (cytologie), Cellules souches mésenchymateuses (métabolisme), Céramiques (composition chimique), Céramiques (métabolisme), Différenciation cellulaire (MeSH), Femelle (MeSH), Matériaux biocompatibles (composition chimique), Matériaux biocompatibles (métabolisme), Ostéogenèse (MeSH), Ovariectomie (MeSH), Porosité (MeSH), Prolifération cellulaire (MeSH), Rat Wistar (MeSH), Rats (MeSH), Récepteur PPAR gamma (génétique), Régulation de l'expression des gènes (MeSH), Sous-unité alpha 1 du facteur CBF (génétique).
MESH :
- composition chimique : Céramiques, Matériaux biocompatibles.
- cytologie : Cellules souches mésenchymateuses.
- génétique : Récepteur PPAR gamma, Sous-unité alpha 1 du facteur CBF.
- métabolisme : Cellules souches mésenchymateuses, Céramiques, Matériaux biocompatibles.
- Adipogenèse, Animaux, Cellules cultivées, Différenciation cellulaire, Femelle, Ostéogenèse, Ovariectomie, Porosité, Prolifération cellulaire, Rat Wistar, Rats, Régulation de l'expression des gènes.

English descriptors

KwdEn :
- Adipogenesis (MeSH), Animals (MeSH), Biocompatible Materials (chemistry), Biocompatible Materials (metabolism), Cell Differentiation (MeSH), Cell Proliferation (MeSH), Cells, Cultured (MeSH), Ceramics (chemistry), Ceramics (metabolism), Core Binding Factor Alpha 1 Subunit (genetics), Female (MeSH), Gene Expression Regulation (MeSH), Mesenchymal Stem Cells (cytology), Mesenchymal Stem Cells (metabolism), Osteogenesis (MeSH), Ovariectomy (MeSH), PPAR gamma (genetics), Porosity (MeSH), Rats (MeSH), Rats, Wistar (MeSH).
MESH :
- chemical , chemistry : Biocompatible Materials.
- chemical , genetics : Core Binding Factor Alpha 1 Subunit, PPAR gamma.
- chemical , metabolism : Biocompatible Materials.
- chemistry : Ceramics.
- cytology : Mesenchymal Stem Cells.
- metabolism : Ceramics, Mesenchymal Stem Cells.
- Adipogenesis, Animals, Cell Differentiation, Cell Proliferation, Cells, Cultured, Female, Gene Expression Regulation, Osteogenesis, Ovariectomy, Porosity, Rats, Rats, Wistar.

Abstract

This study evaluated the effect of mesoporous bioglass (MBG) dissolution on the differentiation of bone marrow mesenchymal stem cells (BMSCs) derived from either sham control or ovariectomized (OVX) rats. MBG was fabricated by evaporation-induced self-assembly method. Cell proliferation was tested by Cell Counting Kit-8 assay, and cytoskeletal morphology was observed by fluorescence microscopy. Osteogenic differentiation was evaluated by alkaline phosphatase (ALP) staining and activity, Alizarin Red staining, while adipogenic differentiation was assessed by Oil Red-O staining. Quantitative real-time PCR and Western blot analysis were taken to evaluate the expression of runt-related transcription factor 2 (Runx2) and proliferator-activated receptor-γ (PPARγ). We found that MBG dissolution (0, 25, 50, 100, 200 µg/mL) was nontoxic to BMSCs growth. Sham and OVX BMSCs exhibited the highest ALP activity in 50 µg/mL of MBG osteogenic dissolution, except that sham BMSCs in 100 µg/mL showed the highest ALP activity on day 14. Runx2 was significantly upregulated after 100 µg/mL of MBG stimulation in sham and OVX BMSCs for 7 and 14 days, except that 25 µg/mL showed highest upregulation effect on OVX BMSCs at day 7. PPARγ was downregulated after MBG stimulation. The protein level of Runx2 from the sham BMSCs group was significantly upregulated after lower doses (25 and 50 µg/mL) of MBG stimulation, whereas PPARγ was downregulated in the sham and OVX BMSCs group. Thus, both the osteogenic and adipogenic abilities of BMSCs were damaged under OVX condition. Moreover, lower concentration of MBG dissolution can promote osteogenesis but inhibit adipogenesis of the sham and OVX BMSCs. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 104A: 3004-3014, 2016.

DOI: 10.1002/jbm.a.35841
PubMed: 27449696
PubMed Central: PMC5995467

Affiliations:

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Le document en format XML

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<term>Animals (MeSH)</term>
<term>Biocompatible Materials (chemistry)</term>
<term>Biocompatible Materials (metabolism)</term>
<term>Cell Differentiation (MeSH)</term>
<term>Cell Proliferation (MeSH)</term>
<term>Cells, Cultured (MeSH)</term>
<term>Ceramics (chemistry)</term>
<term>Ceramics (metabolism)</term>
<term>Core Binding Factor Alpha 1 Subunit (genetics)</term>
<term>Female (MeSH)</term>
<term>Gene Expression Regulation (MeSH)</term>
<term>Mesenchymal Stem Cells (cytology)</term>
<term>Mesenchymal Stem Cells (metabolism)</term>
<term>Osteogenesis (MeSH)</term>
<term>Ovariectomy (MeSH)</term>
<term>PPAR gamma (genetics)</term>
<term>Porosity (MeSH)</term>
<term>Rats (MeSH)</term>
<term>Rats, Wistar (MeSH)</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>Adipogenèse (MeSH)</term>
<term>Animaux (MeSH)</term>
<term>Cellules cultivées (MeSH)</term>
<term>Cellules souches mésenchymateuses (cytologie)</term>
<term>Cellules souches mésenchymateuses (métabolisme)</term>
<term>Céramiques (composition chimique)</term>
<term>Céramiques (métabolisme)</term>
<term>Différenciation cellulaire (MeSH)</term>
<term>Femelle (MeSH)</term>
<term>Matériaux biocompatibles (composition chimique)</term>
<term>Matériaux biocompatibles (métabolisme)</term>
<term>Ostéogenèse (MeSH)</term>
<term>Ovariectomie (MeSH)</term>
<term>Porosité (MeSH)</term>
<term>Prolifération cellulaire (MeSH)</term>
<term>Rat Wistar (MeSH)</term>
<term>Rats (MeSH)</term>
<term>Récepteur PPAR gamma (génétique)</term>
<term>Régulation de l'expression des gènes (MeSH)</term>
<term>Sous-unité alpha 1 du facteur CBF (génétique)</term>
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<keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Biocompatible Materials</term>
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<keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Ceramics</term>
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<keywords scheme="MESH" qualifier="composition chimique" xml:lang="fr"><term>Céramiques</term>
<term>Matériaux biocompatibles</term>
</keywords>
<keywords scheme="MESH" qualifier="cytologie" xml:lang="fr"><term>Cellules souches mésenchymateuses</term>
</keywords>
<keywords scheme="MESH" qualifier="cytology" xml:lang="en"><term>Mesenchymal Stem Cells</term>
</keywords>
<keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Récepteur PPAR gamma</term>
<term>Sous-unité alpha 1 du facteur CBF</term>
</keywords>
<keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Ceramics</term>
<term>Mesenchymal Stem Cells</term>
</keywords>
<keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Cellules souches mésenchymateuses</term>
<term>Céramiques</term>
<term>Matériaux biocompatibles</term>
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<keywords scheme="MESH" xml:lang="en"><term>Adipogenesis</term>
<term>Animals</term>
<term>Cell Differentiation</term>
<term>Cell Proliferation</term>
<term>Cells, Cultured</term>
<term>Female</term>
<term>Gene Expression Regulation</term>
<term>Osteogenesis</term>
<term>Ovariectomy</term>
<term>Porosity</term>
<term>Rats</term>
<term>Rats, Wistar</term>
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<keywords scheme="MESH" xml:lang="fr"><term>Adipogenèse</term>
<term>Animaux</term>
<term>Cellules cultivées</term>
<term>Différenciation cellulaire</term>
<term>Femelle</term>
<term>Ostéogenèse</term>
<term>Ovariectomie</term>
<term>Porosité</term>
<term>Prolifération cellulaire</term>
<term>Rat Wistar</term>
<term>Rats</term>
<term>Régulation de l'expression des gènes</term>
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<front><div type="abstract" xml:lang="en">This study evaluated the effect of mesoporous bioglass (MBG) dissolution on the differentiation of bone marrow mesenchymal stem cells (BMSCs) derived from either sham control or ovariectomized (OVX) rats. MBG was fabricated by evaporation-induced self-assembly method. Cell proliferation was tested by Cell Counting Kit-8 assay, and cytoskeletal morphology was observed by fluorescence microscopy. Osteogenic differentiation was evaluated by alkaline phosphatase (ALP) staining and activity, Alizarin Red staining, while adipogenic differentiation was assessed by Oil Red-O staining. Quantitative real-time PCR and Western blot analysis were taken to evaluate the expression of runt-related transcription factor 2 (Runx2) and proliferator-activated receptor-γ (PPARγ). We found that MBG dissolution (0, 25, 50, 100, 200 µg/mL) was nontoxic to BMSCs growth. Sham and OVX BMSCs exhibited the highest ALP activity in 50 µg/mL of MBG osteogenic dissolution, except that sham BMSCs in 100 µg/mL showed the highest ALP activity on day 14. Runx2 was significantly upregulated after 100 µg/mL of MBG stimulation in sham and OVX BMSCs for 7 and 14 days, except that 25 µg/mL showed highest upregulation effect on OVX BMSCs at day 7. PPARγ was downregulated after MBG stimulation. The protein level of Runx2 from the sham BMSCs group was significantly upregulated after lower doses (25 and 50 µg/mL) of MBG stimulation, whereas PPARγ was downregulated in the sham and OVX BMSCs group. Thus, both the osteogenic and adipogenic abilities of BMSCs were damaged under OVX condition. Moreover, lower concentration of MBG dissolution can promote osteogenesis but inhibit adipogenesis of the sham and OVX BMSCs. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 104A: 3004-3014, 2016.</div>
</front>
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<Day>06</Day>
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<Issue>12</Issue>
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<Month>12</Month>
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<Title>Journal of biomedical materials research. Part A</Title>
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<ArticleTitle>The effect of mesoporous bioglass on osteogenesis and adipogenesis of osteoporotic BMSCs.</ArticleTitle>
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<Abstract><AbstractText>This study evaluated the effect of mesoporous bioglass (MBG) dissolution on the differentiation of bone marrow mesenchymal stem cells (BMSCs) derived from either sham control or ovariectomized (OVX) rats. MBG was fabricated by evaporation-induced self-assembly method. Cell proliferation was tested by Cell Counting Kit-8 assay, and cytoskeletal morphology was observed by fluorescence microscopy. Osteogenic differentiation was evaluated by alkaline phosphatase (ALP) staining and activity, Alizarin Red staining, while adipogenic differentiation was assessed by Oil Red-O staining. Quantitative real-time PCR and Western blot analysis were taken to evaluate the expression of runt-related transcription factor 2 (Runx2) and proliferator-activated receptor-γ (PPARγ). We found that MBG dissolution (0, 25, 50, 100, 200 µg/mL) was nontoxic to BMSCs growth. Sham and OVX BMSCs exhibited the highest ALP activity in 50 µg/mL of MBG osteogenic dissolution, except that sham BMSCs in 100 µg/mL showed the highest ALP activity on day 14. Runx2 was significantly upregulated after 100 µg/mL of MBG stimulation in sham and OVX BMSCs for 7 and 14 days, except that 25 µg/mL showed highest upregulation effect on OVX BMSCs at day 7. PPARγ was downregulated after MBG stimulation. The protein level of Runx2 from the sham BMSCs group was significantly upregulated after lower doses (25 and 50 µg/mL) of MBG stimulation, whereas PPARγ was downregulated in the sham and OVX BMSCs group. Thus, both the osteogenic and adipogenic abilities of BMSCs were damaged under OVX condition. Moreover, lower concentration of MBG dissolution can promote osteogenesis but inhibit adipogenesis of the sham and OVX BMSCs. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 104A: 3004-3014, 2016.</AbstractText>
<CopyrightInformation>© 2016 Wiley Periodicals, Inc.</CopyrightInformation>
</Abstract>
<AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Wu</LastName>
<ForeName>Tao</ForeName>
<Initials>T</Initials>
<AffiliationInfo><Affiliation>The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) & Key Laboratory of Oral Biomedicine Ministry of Education, School & Hospital of Stomatology, Wuhan University, Wuhan, 430079, People's Republic of China.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Cheng</LastName>
<ForeName>Ning</ForeName>
<Initials>N</Initials>
<AffiliationInfo><Affiliation>The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) & Key Laboratory of Oral Biomedicine Ministry of Education, School & Hospital of Stomatology, Wuhan University, Wuhan, 430079, People's Republic of China.</Affiliation>
</AffiliationInfo>
<AffiliationInfo><Affiliation>School of Dentistry, Oral Biology Curriculum, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, 27599.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Xu</LastName>
<ForeName>Chun</ForeName>
<Initials>C</Initials>
<AffiliationInfo><Affiliation>Australian Institute for Bioengineering and Nanotechnology (AIBN), the University of Queensland, Brisbane, QLD, 4067, Australia.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Sun</LastName>
<ForeName>Wei</ForeName>
<Initials>W</Initials>
<AffiliationInfo><Affiliation>The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) & Key Laboratory of Oral Biomedicine Ministry of Education, School & Hospital of Stomatology, Wuhan University, Wuhan, 430079, People's Republic of China.</Affiliation>
</AffiliationInfo>
<AffiliationInfo><Affiliation>Department of Dental Implantology, School and Hospital of Stomatology, Wuhan University, Wuhan, People's Republic of China.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Yu</LastName>
<ForeName>Chengzhong</ForeName>
<Initials>C</Initials>
<AffiliationInfo><Affiliation>Australian Institute for Bioengineering and Nanotechnology (AIBN), the University of Queensland, Brisbane, QLD, 4067, Australia.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Shi</LastName>
<ForeName>Bin</ForeName>
<Initials>B</Initials>
<AffiliationInfo><Affiliation>The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) & Key Laboratory of Oral Biomedicine Ministry of Education, School & Hospital of Stomatology, Wuhan University, Wuhan, 430079, People's Republic of China. shibin_dentist@126.com.</Affiliation>
</AffiliationInfo>
<AffiliationInfo><Affiliation>Department of Dental Implantology, School and Hospital of Stomatology, Wuhan University, Wuhan, People's Republic of China. shibin_dentist@126.com.</Affiliation>
</AffiliationInfo>
</Author>
</AuthorList>
<Language>eng</Language>
<GrantList CompleteYN="Y"><Grant><GrantID>F99 CA223019</GrantID>
<Acronym>CA</Acronym>
<Agency>NCI NIH HHS</Agency>
<Country>United States</Country>
</Grant>
</GrantList>
<PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType>
</PublicationTypeList>
<ArticleDate DateType="Electronic"><Year>2016</Year>
<Month>08</Month>
<Day>05</Day>
</ArticleDate>
</Article>
<MedlineJournalInfo><Country>United States</Country>
<MedlineTA>J Biomed Mater Res A</MedlineTA>
<NlmUniqueID>101234237</NlmUniqueID>
<ISSNLinking>1549-3296</ISSNLinking>
</MedlineJournalInfo>
<ChemicalList><Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D001672">Biocompatible Materials</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="C022107">Bioglass</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D050659">Core Binding Factor Alpha 1 Subunit</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D047495">PPAR gamma</NameOfSubstance>
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<Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="C493717">Runx2 protein, rat</NameOfSubstance>
</Chemical>
</ChemicalList>
<CitationSubset>IM</CitationSubset>
<MeshHeadingList><MeshHeading><DescriptorName UI="D050156" MajorTopicYN="Y">Adipogenesis</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D001672" MajorTopicYN="N">Biocompatible Materials</DescriptorName>
<QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName>
<QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D002454" MajorTopicYN="N">Cell Differentiation</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D049109" MajorTopicYN="N">Cell Proliferation</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D002478" MajorTopicYN="N">Cells, Cultured</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D002516" MajorTopicYN="N">Ceramics</DescriptorName>
<QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName>
<QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D050659" MajorTopicYN="N">Core Binding Factor Alpha 1 Subunit</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D005260" MajorTopicYN="N">Female</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D005786" MajorTopicYN="N">Gene Expression Regulation</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D059630" MajorTopicYN="N">Mesenchymal Stem Cells</DescriptorName>
<QualifierName UI="Q000166" MajorTopicYN="Y">cytology</QualifierName>
<QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D010012" MajorTopicYN="Y">Osteogenesis</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D010052" MajorTopicYN="N">Ovariectomy</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D047495" MajorTopicYN="N">PPAR gamma</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D016062" MajorTopicYN="N">Porosity</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D051381" MajorTopicYN="N">Rats</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D017208" MajorTopicYN="N">Rats, Wistar</DescriptorName>
</MeshHeading>
</MeshHeadingList>
<KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="Y">adipogenesis</Keyword>
<Keyword MajorTopicYN="Y">bone marrow mesenchymal stem cells (BMSCs)</Keyword>
<Keyword MajorTopicYN="Y">mesoporous bioglass (MBG)</Keyword>
<Keyword MajorTopicYN="Y">osteogenesis</Keyword>
<Keyword MajorTopicYN="Y">osteoporosis</Keyword>
</KeywordList>
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<Month>04</Month>
<Day>16</Day>
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<PubMedPubDate PubStatus="revised"><Year>2016</Year>
<Month>06</Month>
<Day>21</Day>
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<PubMedPubDate PubStatus="accepted"><Year>2016</Year>
<Month>07</Month>
<Day>21</Day>
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<Month>10</Month>
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	Serveur d'exploration sur le suicide chez les dentistes
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The effect of mesoporous bioglass on osteogenesis and adipogenesis of osteoporotic BMSCs.

The effect of mesoporous bioglass on osteogenesis and adipogenesis of osteoporotic BMSCs.

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